HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

Detection of DNA lesions induced by chemical mutagens by the single cell electrophoresis (Comet) assay. 1. Relationship between the onset of DNA damage and the characteristics of mutagens.

Abstract
We evaluated the relationship between the onset of DNA damage and the characteristics of 5 model chemical mutagens with the single-cell gel electrophoresis (SCG) assay using L5178Y mouse lymphoma cells. We treated the cells with each chemical for 3 h and sampled them 0.21, and 45 h after treatment. DNA damage induced by UV mimetic mutagens MMS and MNU, and X-ray mimetic mutagen BLM was observed just after treatment, crosslinking agent MMC-induced DNA damage was detected 21 h after treatment, and 6-MP as an inhibitor of DNA synthesis did not induce DNA damage at any sampling time. These results suggest that the SCG assay detects DNA lesions just after treatment with UV and X-ray mimetic mutagens, but needs a waiting period after treatment with crosslinking agents.
AuthorsY Miyamae, K Zaizen, K Ohara, Y Mine, Y F Sasaki
JournalMutation research (Mutat Res) Vol. 415 Issue 3 Pg. 229-35 (Jul 31 1998) ISSN: 0027-5107 [Print] Netherlands
PMID9714818 (Publication Type: Corrected and Republished Article, Journal Article)
Chemical References
  • Mutagens
Topics
  • Animals
  • Cell Movement (drug effects)
  • Cell Survival (drug effects)
  • DNA Damage
  • Electrophoresis, Agar Gel
  • Leukemia L5178
  • Mice
  • Mutagenicity Tests
  • Mutagens (toxicity)
  • Time Factors
  • Tumor Cells, Cultured

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: