Abstract |
We evaluated the relationship between the onset of DNA damage and the characteristics of 5 model chemical mutagens with the single-cell gel electrophoresis (SCG) assay using L5178Y mouse lymphoma cells. We treated the cells with each chemical for 3 h and sampled them 0.21, and 45 h after treatment. DNA damage induced by UV mimetic mutagens MMS and MNU, and X-ray mimetic mutagen BLM was observed just after treatment, crosslinking agent MMC-induced DNA damage was detected 21 h after treatment, and 6-MP as an inhibitor of DNA synthesis did not induce DNA damage at any sampling time. These results suggest that the SCG assay detects DNA lesions just after treatment with UV and X-ray mimetic mutagens, but needs a waiting period after treatment with crosslinking agents.
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Authors | Y Miyamae, K Zaizen, K Ohara, Y Mine, Y F Sasaki |
Journal | Mutation research
(Mutat Res)
Vol. 415
Issue 3
Pg. 229-35
(Jul 31 1998)
ISSN: 0027-5107 [Print] Netherlands |
PMID | 9714818
(Publication Type: Corrected and Republished Article, Journal Article)
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Chemical References |
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Topics |
- Animals
- Cell Movement
(drug effects)
- Cell Survival
(drug effects)
- DNA Damage
- Electrophoresis, Agar Gel
- Leukemia L5178
- Mice
- Mutagenicity Tests
- Mutagens
(toxicity)
- Time Factors
- Tumor Cells, Cultured
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