De novo protein synthesis is essential for thermotolerance acquisition in a Saccharomyces cerevisiae trehalose synthase mutant.

Abstract

Heat shock (25 degrees C to 37 degrees C for 30 min) acquisition of thermotolerance (at 50 degrees C) was observed in a yeast trehalose synthase mutant and the corresponding control strain. The acquisition of thermotolerance in the control strain was maintained for a significantly longer time than in the trehalose synthase mutant. The heat shock was associated with the synthesis of specific heat shock proteins and, in the case of the control strain, also trehalose accumulation. Inhibition of protein synthesis during the heat shock totally abolished acquisition of thermotolerance in both strains but not trehalose accumulation in the control. It was concluded that trehalose may only be required for prolonged stress protection while heat shock proteins are required for heat shock acquisition of thermotolerance.

• Authors: C Gross, K Watson
• Journal: Biochemistry and molecular biology international (Biochem Mol Biol Int) Vol. 45 Issue 4 Pg. 663-71 (Jul 1998) ISSN: 1039-9712 [Print] England
• PMID: 9713688 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fungal Proteins
• Heat-Shock Proteins
• Trehalose
• Glucosyltransferases
• trehalose synthase

Topics

• Fungal Proteins (biosynthesis)
• Glucosyltransferases (genetics, metabolism)
• Heat-Shock Proteins (biosynthesis)
• Heat-Shock Response
• Hot Temperature
• Mutation
• Saccharomyces cerevisiae (enzymology, genetics, metabolism)
• Trehalose (biosynthesis)