Apoptosis of rat kidney cells after 241-americium administration.

Abstract

Tumors induction by americium is well known but there are no data on the biological effects of this radionucleide at subcellular level. In order to study the possible ultrastructural lesions induced by this element, a group of rats were injected with 241-Americium-citrate (9 kBq), once a week for five weeks and sacrificed 7 days after the last injection. We describe the alterations observed in the cortex kidney using cytochemical (TUNEL reaction) and histochemical (PAS staining) methods for light microscopy as well as electron microscopy techniques. Various types of lesions were detected: condensation of nuclear chromatine, fragmentation of the nuclei, swollen mitochondria, disappearance of mitochondrial crests and skrinking of the cytoplasm. This study clearly demonstrated the induction of apoptosis by americium in rat cortex kidney cells.

• Authors: L Labéjof, J P Berry, P Duchambon, J L Poncy, P Galle
• Journal: Anticancer research (Anticancer Res) 1998 Jul-Aug Vol. 18 Issue 4A Pg. 2409-14 ISSN: 0250-7005 [Print] Greece
• PMID: 9703887 (Publication Type: Journal Article)

Chemical References

• Americium

Topics

• Americium (pharmacology)
• Animals
• Apoptosis (radiation effects)
• Cell Nucleus (radiation effects, ultrastructure)
• DNA Fragmentation
• Kidney Cortex (pathology, radiation effects, ultrastructure)
• Kidney Tubules, Distal (pathology, radiation effects, ultrastructure)
• Rats
• Rats, Sprague-Dawley