Abstract |
We have cloned and sequenced a tumor rejection antigen from a highly immunogenic rat histiocytoma, AK-5. Recombinant antigen induced an antibody response in syngeneic hosts, and the immunized animals showed significant resistance to AK-5 tumor challenge, where growth of the tumor was retarded. Autologous anti-AK-5 antibody, as well as the specific monoclonal antibodies raised against whole AK-5 cells, recognized the recombinant protein. Similarly, recombinant antigen was able to neutralize antitumor antibody in a complement-mediated lysis assay. Antibodies raised against the fusion protein recognized a 60-kDa protein from AK-5 cell extracts. A cDNA sequence analysis revealed significant homology with mouse thymus (81%), lymph node (83%), and human brain (71%) cDNAs; however, the function of these genes is not known. These observations suggest the recombinant protein to be a strong candidate for the tumor rejection antigen that is involved in the spontaneous regression of the AK-5 tumor in syngeneic hosts.
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Authors | T Muralikrishna, Z Begum, C V Swamy, A Khar |
Journal | DNA and cell biology
(DNA Cell Biol)
Vol. 17
Issue 7
Pg. 603-12
(Jul 1998)
ISSN: 1044-5498 [Print] United States |
PMID | 9703019
(Publication Type: Journal Article)
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Chemical References |
- AK-5 antigen
- Antibodies, Neoplasm
- Antigens, Neoplasm
- DNA, Complementary
- DNA, Neoplasm
- Recombinant Fusion Proteins
- Glutathione Transferase
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Topics |
- Amino Acid Sequence
- Animals
- Antibodies, Neoplasm
(genetics, immunology)
- Antigens, Neoplasm
(analysis, genetics, immunology)
- Base Sequence
- Cloning, Molecular
- DNA, Complementary
(chemistry, genetics)
- DNA, Neoplasm
(genetics, isolation & purification)
- Female
- Genes
(genetics)
- Glutathione Transferase
(immunology)
- Male
- Molecular Sequence Data
- Neutralization Tests
- Rats
- Rats, Wistar
- Recombinant Fusion Proteins
(genetics, immunology)
- Sequence Alignment
- Sequence Analysis, DNA
- Sequence Homology, Amino Acid
- Sequence Homology, Nucleic Acid
- Tumor Cells, Cultured
(chemistry, immunology, metabolism)
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