A unique form of proliferating cell nuclear antigen is present in malignant breast cells.

Abstract

Despite extensive research efforts to identify unique molecular alterations in breast cancer, to date, no characteristic has emerged that correlates exclusively with malignancy. Recently, it has been shown that the multiprotein DNA replication complex (synthesome) from breast cancer cells has a significantly decreased replication fidelity compared to that of nonmalignant breast cells. Proliferating cell nuclear antigen (PCNA) functions in DNA replication and DNA repair and is a component of the synthesome. Using two-dimensional PAGE analysis, we have identified a novel form of PCNA in malignant breast cells. This unique form is not the result of a genetic alteration, as demonstrated by DNA sequence analysis of the PCNA gene from malignant and nonmalignant breast cells. This novel form is most likely the result of an alteration in the post-translational modification of PCNA in malignant breast cells. These findings are significant in that it is now possible to link changes in the fidelity of DNA replication with a specific alteration of a component of the DNA synthetic apparatus of breast cancer cells. The novel form of PCNA may prove to be a new signature for malignant breast cells.

• Authors: P E Bechtel, R J Hickey, L Schnaper, J W Sekowski, B J Long, R Freund, N Liu, C Rodriguez-Valenzuela, L H Malkas
• Journal: Cancer research (Cancer Res) Vol. 58 Issue 15 Pg. 3264-9 (Aug 01 1998) ISSN: 0008-5472 [Print] United States
• PMID: 9699653 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA, Neoplasm
• Proliferating Cell Nuclear Antigen

Topics

• Animals
• Base Sequence
• Breast Neoplasms (genetics, metabolism, pathology)
• Cell Division (physiology)
• DNA, Neoplasm (biosynthesis)
• Humans
• Mice
• Molecular Sequence Data
• Mutation
• Proliferating Cell Nuclear Antigen (biosynthesis, genetics)
• Tumor Cells, Cultured