Screening of N-acylneuraminic acids in serum and tissue specimens of mouse C57BI with Lewis' lung cancer by high-performance liquid chromatography.

Abstract

Serum and tissue specimens from healthy C57BI mice and from mice with Lewis' lung cancer after metastasis were analyzed for N-acetyl- and N-glycolylneuraminic acid by high-performance liquid chromatography. Both neuraminic acids were present, while N-glycolylneuraminic acid was the predominant sialic acid in all tissues. Samples from mice with metastatic cancer showed a significant increase (67-200%) of total sialic acids mainly as a result of increased N-glycolylneuraminic acid synthesis. These results suggest that cancer metastasis in various tissues is closely associated with increased synthesis of the predominant neuraminic acid and may help to understand the underlying mechanisms of tumor development.

• Authors: E Makatsori, K Fermani, A Aletras, N K Karamanos, T Tsegenidis
• Journal: Journal of chromatography. B, Biomedical sciences and applications (J Chromatogr B Biomed Sci Appl) Vol. 712 Issue 1-2 Pg. 23-9 (Aug 07 1998) ISSN: 1387-2273 [Print] Netherlands
• PMID: 9698225 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Neuraminic Acids
• N-glycolylneuraminic acid
• N-Acetylneuraminic Acid

Topics

• Animals
• Biomarkers, Tumor (analysis, blood)
• Carcinoma, Lewis Lung (blood, chemistry, secondary)
• Chromatography, High Pressure Liquid
• Hindlimb
• Male
• Mice
• Mice, Inbred C57BL
• Muscle, Skeletal (chemistry)
• N-Acetylneuraminic Acid (analysis, blood)
• Neoplasm Transplantation
• Neuraminic Acids (analysis, blood)