The gene encoding the granulocyte colony-stimulating factor receptor is a target for deregulation in pre-B ALL by the t(1;19)-specific oncoprotein E2A-Pbx1.

Abstract

Approximately 25-30% of childhood pre-B cell acute lymphoblastic leukemias (pre-B ALL) is characterized by the presence of a (1;19)(q23;p13.3) translocation. The presence of this translocation is generally accompanied by a poor prognosis. The chimeric gene resulting from this chromosomal rearrangement encodes a hybrid transcription factor, E2A-Pbx1. In an attempt to delineate the genetic cascade initiated by E2A-Pbx1, we sought to identify genes that are deregulated by this transcription factor in t(1;19) pre-B ALL. We show here that the gene encoding the granulocyte colony-stimulating factor receptor (G-CSFr) is specifically upregulated in pre-B cells expressing E2A-Pbx1. G-CSFr is also expressed in cell lines established from t(1;19) pre-B cell leukemia and on primary t(1;19) tumor cells, but not on control cells. These data indicate that G-CSFr gene is a target for deregulation by E2A-Pbx1.

• Authors: W B de Lau, J Hurenkamp, P Berendes, I P Touw, H C Clevers, M A van Dijk
• Journal: Oncogene (Oncogene) Vol. 17 Issue 4 Pg. 503-10 (Jul 30 1998) ISSN: 0950-9232 [Print] England
• PMID: 9696044 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Homeodomain Proteins
• Oncogene Proteins, Fusion
• Receptors, Granulocyte Colony-Stimulating Factor
• E2A-Pbx1 fusion protein

Topics

• B-Lymphocytes
• Burkitt Lymphoma (genetics, metabolism)
• Chromosomes, Human, Pair 1
• Chromosomes, Human, Pair 19
• Gene Expression Regulation
• Hematopoietic Stem Cells
• Homeodomain Proteins (genetics, metabolism)
• Humans
• Oncogene Proteins, Fusion (genetics, metabolism)
• Receptors, Granulocyte Colony-Stimulating Factor (genetics, physiology)
• Translocation, Genetic
• Tumor Cells, Cultured