DOC-2/hDab2, a candidate tumor suppressor gene involved in the development of gestational trophoblastic diseases.

Abstract	Gestational trophoblastic diseases comprise a spectrum of interrelated diseases including partial mole, complete mole and gestational choriocarcinoma. Using reverse transcriptase PCR (RT-PCR) analysis, we identified higher levels of DOC-2/hDab2 expression in the normal trophoblast cells in culture than in choriocarcinoma cell lines. Subsequent study using immunohistochemistry showed high levels of DOC-2/hDab2 protein expression in normal trophoblast tissues but significantly lower levels of expression in gestational trophoblastic disease tissues, particularly in complete mole and choriocarcinoma. When DOC-2/hDab2 was transfected into the choriocarcinoma cell lines, Jar, JEG and BeWo, the stable transfectants showed significantly reduced growth rate in culture. These data suggest that down regulation of DOC-2/hDab2 may play an important role in the development of gestational trophoblastic diseases.
Authors	V Fulop, C V Colitti, D Genest, R S Berkowitz, G K Yiu, S W Ng, J Szepesi, S C Mok
Journal	Oncogene (Oncogene) Vol. 17 Issue 4 Pg. 419-24 (Jul 30 1998) ISSN: 0950-9232 [Print] England
PMID	9696034 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S., Research Support, U.S. Gov't, P.H.S.)
Chemical References	Adaptor Proteins, Signal Transducing Adaptor Proteins, Vesicular Transport Apoptosis Regulatory Proteins Coloring Agents DAB2 protein, human Proteins Tetrazolium Salts Thiazoles Tumor Suppressor Proteins thiazolyl blue
Topics	Adaptor Proteins, Signal Transducing Adaptor Proteins, Vesicular Transport Amino Acid Sequence Apoptosis Regulatory Proteins Blotting, Western Cell Division Cell Line Choriocarcinoma (genetics, metabolism, pathology) Coloring Agents Female Genes, Tumor Suppressor Humans Hydatidiform Mole (genetics, metabolism, pathology) Hydatidiform Mole, Invasive (genetics, metabolism, pathology) Immunoenzyme Techniques Molecular Sequence Data Polymerase Chain Reaction (methods) Pregnancy Protein Biosynthesis Proteins (genetics) Tetrazolium Salts Thiazoles Transfection Trophoblastic Neoplasms (genetics) Tumor Cells, Cultured Tumor Suppressor Proteins Uterine Neoplasms (genetics, metabolism, pathology)

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