Evaluation of boron neutron capture effects in cell culture using sulforhodamine-B assay and a colony assay.

Abstract

PURPOSE:

The purpose of this study was to find an in vitro method for determining the cytotoxicity of boronated drugs as well as their potential suitability for neutron capture therapy.

MATERIALS AND METHODS:

The survival of human melanoma cells has been determined by a colony assay and the sulforhodamine-B assay after X-irradiation and irradiation with fast d(14) + Be-neutrons using the boronated compound borocaptate sodium (BSH). The cytotoxic effects of BSH have been studied using both methods.

RESULTS:

Under well-defined experimental conditions, and after a sufficient amount of time for the expression of radiation damage, the results of the sulforhodamine-B assay are qualitatively comparable with the results of the colony assay.

CONCLUSION:

The sulforhodamine-B assay is suitable for the screening of compounds for potential use in neutron capture therapy because it is a fast and efficient method that is reproducible and technically advantageous.

• Authors: A Wittig, W Sauerwein, F Pöller, C Fuhrmann, K Hideghéty, C Streffer
• Journal: International journal of radiation biology (Int J Radiat Biol) Vol. 73 Issue 6 Pg. 679-90 (Jun 1998) ISSN: 0955-3002 [Print] England
• PMID: 9690686 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Fluorescent Dyes
• Rhodamines
• lissamine rhodamine B

Topics

• Boron Neutron Capture Therapy
• Cell Survival (radiation effects)
• Dose-Response Relationship, Radiation
• Fast Neutrons
• Fluorescent Dyes
• Humans
• Melanoma (pathology, radiotherapy)
• Rhodamines
• Tumor Cells, Cultured
• Tumor Stem Cell Assay
• X-Rays