Abstract | BACKGROUND: METHODS: Both kidneys were removed from male Lewis rats and re-implanted the left kidney after 30 minutes of cold ischemia time. The warm ischemia time was 60 minutes. Sham operated, uninephrectomized animals served as controls for renal function and histology. ICAM-1 antisense ODN (5 mg/kg), reverse ODN, or saline-vehicle were administered to donor animals i.v. six hours before autotransplantation. Glomerular filtration rate ( insulin clearance), and serum creatinine concentrations were measured 24 hours post- transplantation. Tubular necrosis severity was assessed by histological grading scale. ICAM-1 expression was determined by immunohistochemistry and Western blot. RESULTS: Antisense ODN decreased ICAM-1 expression and leukocyte infiltration significant. Antisense ODN-treated animals showed significantly less tubular necrosis, than controls. Serum creatinine of antisense ODN-treated animals (N = 6) was 0.55 +/- 0.02 mg/dl compared to 1.92 +/- 0.07 mg/dl in reverse ODN-treated controls (N = 6; P < 0.01), 24 hours after transplantation. Antisense ODN-treated animals had normal GFR (0.93 +/- 0.07 ml/min/kidney wt) compared to sham-operated animals (0.95 +/- 0.09 ml/min/kidney wt), while autotransplanted animals treated with reverse ODN or saline-vehicle were all anuric. The ischemia-reperfusion-induced up-regulation of MHC class II was totally prevented by antisense ODN. CONCLUSIONS:
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Authors | D Dragun, S G Tullius, J K Park, C Maasch, I Lukitsch, A Lippoldt, V Gross, F C Luft, H Haller |
Journal | Kidney international
(Kidney Int)
Vol. 54
Issue 2
Pg. 590-602
(Aug 1998)
ISSN: 0085-2538 [Print] United States |
PMID | 9690227
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Oligonucleotides, Antisense
- Intercellular Adhesion Molecule-1
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Topics |
- Animals
- Intercellular Adhesion Molecule-1
(physiology)
- Kidney
(pathology)
- Kidney Transplantation
- Male
- Oligonucleotides, Antisense
(therapeutic use)
- Rats
- Rats, Inbred Lew
- Reperfusion Injury
(prevention & control)
- Transplantation, Autologous
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