Abstract |
The purpose of this prospective study was to compare two different milk preparation methods to assay for the presence of Mycobacterium bovis by PCR. Detection by a C18-carboxypropylbetaine (CB-18)-based sample processing method was compared to extraction of DNA from milk with glass beads. Samples from 17 skin test-positive cattle were analyzed. Following CB-18 processing and glass bead extraction, the sensitivity of IS6110-based PCR was 94.1 and 58.8%, respectively (P < 0.025). Because CB-18 processing will permit the proficient use of PCR for diagnosis and surveillance of bovine tuberculosis, it will contribute to the more efficient detection and control of tuberculosis.
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Authors | B J Cornejo, A Sahagún-Ruiz, F Suárez-Güemes, C G Thornton, T A Ficht, L G Adams |
Journal | Applied and environmental microbiology
(Appl Environ Microbiol)
Vol. 64
Issue 8
Pg. 3099-101
(Aug 1998)
ISSN: 0099-2240 [Print] United States |
PMID | 9687483
(Publication Type: Comparative Study, Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
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Topics |
- Animals
- Bacteriological Techniques
- Betaine
(analogs & derivatives)
- Cattle
- DNA, Bacterial
(isolation & purification)
- Glass
- Microspheres
- Milk
(microbiology)
- Mycobacterium bovis
(genetics, isolation & purification)
- Polymerase Chain Reaction
(methods)
- Prospective Studies
- Sensitivity and Specificity
- Tuberculin Test
(veterinary)
- Tuberculosis, Bovine
(diagnosis)
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