Isolation and characterization of a bacterium possessing a novel aldoxime-dehydration activity and nitrile-degrading enzymes.

Abstract

A bacterial strain capable of utilizing E-pyridine-3-aldoxime as a nitrogen source was isolated from soil after a 4-month acclimation period and was identified as Rhodococcus sp. The strain contained a novel aldoxime dehydration activity that catalyzed a stoichiometric dehydration of E-pyridine-3-aldoxime to form 3-cyanopyridine. The enzyme activity was induced by various aldoximes and nitriles. The strain metabolized the aldoxime as follows: E-pyridine-3-aldoxime was dehydrated to form 3-cyanopyridine, which was converted to nicotinamide by a nitrile hydratase, and the nicotinamide was successively hydrolyzed to nicotinic acid by an amidase.

• Authors: Y Kato, R Ooi, Y Asano
• Journal: Archives of microbiology (Arch Microbiol) Vol. 170 Issue 2 Pg. 85-90 (Aug 1998) ISSN: 0302-8933 [Print] Germany
• PMID: 9683644 (Publication Type: Journal Article)

Chemical References

• Bacterial Proteins
• Nitriles
• Oximes
• Pyridines
• Niacinamide
• Amidohydrolases
• 3-cyanopyridine

Topics

• Amidohydrolases (metabolism)
• Bacterial Proteins (metabolism)
• Enzyme Induction (physiology)
• Kinetics
• Niacinamide (metabolism)
• Nitriles (metabolism)
• Oximes (metabolism)
• Pyridines (metabolism)
• Rhodococcus (enzymology)
• Soil Microbiology