Cloning of human ENC-1 and evaluation of its expression and regulation in nervous system tumors.

Abstract

We recently identified and characterized a novel murine gene, ENC-1, that is expressed primarily in the nervous system and encodes an actin-binding protein. To gain insight into a potential role for ENC-1 gene in the processes of cell differentiation and malignant transformation in the human nervous system, we first cloned and characterized the human homologue of ENC-1. The human ENC-1 gene appeared to be highly expressed in adult brain and spinal cord, and in a number of cell lines derived from nervous system tumors we detected low steady-state levels of ENC-1 mRNA. We used a neuroblastoma differentiation model, the retinoic acid-induced neuronal differentiation of SMS-KCNR cells, to study the regulation of the ENC-1 gene during neural crest cell differentiation. We found that the expression of ENC-1 increased dramatically in the differentiated SMS-KCNR cells as compared to control undifferentiated cells. These results suggest that ENC-1 expression plays a role during differentiation of neural crest cells and may be down regulated in neuroblastoma tumors.

• Authors: M C Hernandez, P J Andres-Barquin, I Holt, M A Israel
• Journal: Experimental cell research (Exp Cell Res) Vol. 242 Issue 2 Pg. 470-7 (Aug 01 1998) ISSN: 0014-4827 [Print] United States
• PMID: 9683534 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
• Copyright: Copyright 1998 Academic Press.

Chemical References

• Antineoplastic Agents
• DNA, Complementary
• Microfilament Proteins
• Neuropeptides
• Nuclear Proteins
• RNA, Messenger
• ectodermal-neural cortex 1 protein
• Tretinoin
• Bucladesine

Topics

• Amino Acid Sequence
• Antineoplastic Agents (pharmacology)
• Base Sequence
• Blotting, Northern
• Brain (metabolism)
• Bucladesine (pharmacology)
• Cell Differentiation (drug effects, genetics)
• Cell Division (drug effects)
• Central Nervous System (metabolism)
• Cerebral Cortex (metabolism)
• Cloning, Molecular
• DNA, Complementary (chemistry, genetics)
• Gene Expression (drug effects, genetics)
• Gene Expression Regulation
• HL-60 Cells (cytology, drug effects, metabolism)
• Humans
• Microfilament Proteins (genetics, physiology)
• Molecular Sequence Data
• Nervous System Neoplasms (genetics, pathology)
• Neuropeptides
• Nuclear Proteins
• Pancreas (metabolism)
• RNA, Messenger (drug effects, metabolism)
• Sequence Analysis, DNA
• Spinal Cord (metabolism)
• Tissue Distribution
• Tretinoin (pharmacology)
• Tumor Cells, Cultured (cytology, drug effects, metabolism)