Theileria parasite infection in East Asia and control of the disease.

Abstract	Bovine piroplasmosis caused by Theileria sergenti is a major cause of economic loss in grazing cattle in Japan. We found that parasite stocks and isolates consist of genetically and antigenically mixed populations. To differentiate among parasite populations bearing 3 allelic forms of p32/34, an immunodominant piroplasmin surface protein, 3 sets of oligonucleotide primers were designed to amplify either of 3 alleles of T. sergenti/T. buffeli/T. orientalis by polymerase chain reaction (PCR). By using this allele-specific PCR, we found that in East Asia the majority of bovines infected with benign Theileria parasites harbored mixed parasite population. As a possible means of controlling Theileria sergenti infection, we produced 2 candidate vaccines; a bactilovirus expressed recombinant p32 and a synthetic peptide containing of Lys-Glu Lys (KEK) motif. Immunization with either of two candidates resulted in lower parasitemia and reduced the severity of clinical symptoms as compared to control calves.
Authors	M Onuma, T Kakuda, C Sugimoto
Journal	Comparative immunology, microbiology and infectious diseases (Comp Immunol Microbiol Infect Dis) Vol. 21 Issue 3 Pg. 165-77 (Jul 1998) ISSN: 0147-9571 [Print] England
PMID	9681240 (Publication Type: Journal Article, Review)
Chemical References	DNA Primers DNA, Protozoan Membrane Proteins Protozoan Proteins Protozoan Vaccines Vaccines, Synthetic piroplasm surface protein p32, Theileria sergenti
Topics	Alleles Amino Acid Sequence Animals Australia Base Sequence Cattle DNA Primers (chemistry) DNA, Protozoan (chemistry) Asia, Eastern Genetic Variation Host-Parasite Interactions Membrane Proteins (chemistry, genetics, immunology) Molecular Sequence Data Polymerase Chain Reaction (veterinary) Protozoan Proteins (chemistry, genetics, immunology) Protozoan Vaccines (biosynthesis, immunology) Theileria (classification, genetics, immunology) Theileriasis (parasitology, prevention & control) Vaccination (veterinary) Vaccines, Synthetic (immunology)

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