Partial purification of a double-stranded RNA specific ribonuclease (RNAse D) from Krebs II ascites cells.

Abstract

In a search for eucaryotic enzymes which might process the heterogenous nuclear RNA (HnRNA) from animal cells into messenger RNA, a ribonuclease called RNAse D analogous to E. coli RNAse III in its ability to cleave specifically synthetic or viral double-stranded polyribonucleotides has been detected and extensively purified from the cytosol of Krebs II mouse ascites cells. The purification procedure involved cellular fractionation followed by DEAE-and CM-cellulose chromatography and resulted in an RNAas D preparation contaminated with trace amounts of single-strand specific RNAse (equivalent to less than 0.3 ng per ml) as assayed against poly (rC). Significant levels of RNAse H activity against poly (rA)-poly (dT) were still present in these preparations.

• Authors: J Rech, G Cathala, P Jeanteur
• Journal: Nucleic acids research (Nucleic Acids Res) Vol. 3 Issue 8 Pg. 2055-65 (Aug 1976) ISSN: 0305-1048 [Print] England
• PMID: 967689 (Publication Type: Journal Article)

Chemical References

• Ribonucleases

Topics

• Animals
• Carcinoma, Krebs 2 (enzymology)
• Cell Nucleus
• Kinetics
• Mice
• Ribonucleases (isolation & purification, metabolism)
• Subcellular Fractions (enzymology)