Effect of Sulfo Lewis C on smoke inhalation injury in an ovine model.

Abstract

OBJECTIVE:

To evaluate the effect of Sulfo Lewis C (SO3-3âGal1-3GlcNAc-O(CH2)8-COOMe), a putative ligand of selectins, on smoke inhalation injury.

DESIGN:

Prospective animal study with concurrent controls.

SETTING:

An animal laboratory.

SUBJECTS:

Twelve 1-yr-old female sheep, weighing 24 to 33 kg.

INTERVENTIONS:

Twelve sheep received nine exposure units of smoke generated by thermolysis of pine woodchips (80 g). Group 1 (n = 6) was untreated. Group 2 (n = 6) was treated with an intravenous infusion of Sulfo Lewis C after smoke exposure. Animals were killed 48 hrs after injury.

MEASUREMENTS AND MAIN RESULTS:

Cardiopulmonary variables and blood gases were measured serially. Granulocyte free-radical production was measured before smoke exposure and at 4 and 48 hrs after injury. Ventilation/perfusion distribution (VA/Q) was analyzed using the multiple inert gas elimination technique. Granulocyte free-radical production was increased after smoke exposure in both groups. Oxygenation was significantly improved by the administration of Sulfo Lewis C. VA/Q analysis demonstrated significantly less blood flow to low VA/Q lung segments in treated animals.

CONCLUSIONS:

Selectin blockade attenuated lung injury after smoke exposure. These data support the hypothesis that neutrophils play a pivotal role in smoke inhalation injury.

• Authors: O Tasaki, D W Mozingo, S Ishihara, W W Brinkley, A A Johnson, R H Smith, O Srivastava, A D Mason Jr, B A Pruitt Jr, W G Cioffi Jr
• Journal: Critical care medicine (Crit Care Med) Vol. 26 Issue 7 Pg. 1238-43 (Jul 1998) ISSN: 0090-3493 [Print] United States
• PMID: 9671375 (Publication Type: Journal Article, Research Support, U.S. Gov't, Non-P.H.S.)

Chemical References

• Lewis Blood Group Antigens
• Oligosaccharides
• Selectins
• sulfo Lewis C

Topics

• Animals
• Female
• Lewis Blood Group Antigens
• Microcirculation
• Neutrophils (drug effects, metabolism)
• Oligosaccharides (therapeutic use)
• Prospective Studies
• Respiratory Insufficiency (blood, drug therapy, etiology)
• Selectins (drug effects, metabolism)
• Sheep
• Smoke Inhalation Injury (blood, complications)
• Time Factors