Beta-carotene hydroxylase gene from the cyanobacterium Synechocystis sp. PCC6803.

Abstract

The ORF sll1468 of Synechocystis sp. PCC6803 was identified as a gene for beta-carotene hydroxylase by functional complementation in a beta-carotene-producing Escherichia coli. The gene product of ORF sll1468 added hydroxyl groups to the beta-ionone rings of beta-carotene (beta, beta-carotene) to form zeaxanthin (beta, beta-carotene-3,3'-diol). This newly identified beta-carotene hydroxylase does not show overall amino acid sequence similarity to the known beta-carotene hydroxylases. However, it showed significant sequence similarity to beta-carotene ketolases of marine bacteria and a green alga.

• Authors: K Masamoto, N Misawa, T Kaneko, R Kikuno, H Toh
• Journal: Plant & cell physiology (Plant Cell Physiol) Vol. 39 Issue 5 Pg. 560-4 (May 1998) ISSN: 0032-0781 [Print] Japan
• PMID: 9664719 (Publication Type: Comparative Study, Journal Article)

Chemical References

• Bacterial Proteins
• Carotenoids
• Mixed Function Oxygenases
• Oxygenases
• beta-carotene ketolase
• beta-carotene hydroxylase

Topics

• Amino Acid Sequence
• Bacterial Proteins (genetics)
• Carotenoids (biosynthesis)
• Cyanobacteria (enzymology, genetics, metabolism)
• Evolution, Molecular
• Genes, Bacterial
• Mixed Function Oxygenases (genetics, metabolism)
• Molecular Sequence Data
• Oxygenases (genetics)
• Sequence Homology, Amino Acid