Mild acid stress as a differentiation trigger in Trypanosoma brucei.

Abstract	In vitro differentiation of Trypanosoma brucei from the bloodstream to the procyclic form is efficiently induced by the combination of cold shock from 37 to 27 degrees C and the addition of citrate/cis-aconitate (CCA) to the incubation medium. Here it is reported that exposure of pleomorphic bloodstream trypanosomes to mild acidic conditions (pH 5.5 for 2 h at 37 degrees C) not only accelerated the process of morphological transformation from long slender and intermediate to short stumpy bloodstream forms but also allowed their subsequent differentiation into procyclic forms even in the absence of CCA. This process appeared to involve the glycosylphosphatidylinositol-specific phospholipase C (GPI-PLC), since null GPI-PLC mutants (PLC-) appeared to be largely refractory to acid stress-induced differentiation. However, an effective response was restored upon reintegration of the GPI-PLC gene in the genome (PLC+).
Authors	S Rolin, J Hancocq-Quertier, F Paturiaux-Hanocq, D P Nolan, E Pays
Journal	Molecular and biochemical parasitology (Mol Biochem Parasitol) Vol. 93 Issue 2 Pg. 251-62 (Jun 01 1998) ISSN: 0166-6851 [Print] Netherlands
PMID	9662709 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Chemical References	Culture Media Membrane Glycoproteins Protozoan Proteins Variant Surface Glycoproteins, Trypanosoma procyclic acidic repetitive protein, Trypanosoma Citric Acid Aconitic Acid Dihydrolipoamide Dehydrogenase Type C Phospholipases Phosphatidylinositol Diacylglycerol-Lyase Glycosylphosphatidylinositol Diacylglycerol-Lyase
Topics	Aconitic Acid (pharmacology) Animals Citric Acid (pharmacology) Culture Media Dihydrolipoamide Dehydrogenase (metabolism) Genes, Protozoan Glycosylphosphatidylinositol Diacylglycerol-Lyase Hydrogen-Ion Concentration Membrane Glycoproteins (biosynthesis) Mice Mutation Phosphatidylinositol Diacylglycerol-Lyase Protozoan Proteins Trypanosoma brucei brucei (cytology, genetics, growth & development, metabolism) Type C Phospholipases (genetics, metabolism) Variant Surface Glycoproteins, Trypanosoma (analysis)

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