Molecular cloning of xSRC-3, a novel transcription coactivator from Xenopus, that is related to AIB1, p/CIP, and TIF2.

Abstract

Nuclear receptors regulate transcription by binding to specific DNA response elements of target genes. Herein, we report the molecular cloning and characterization of a novel Xenopus cDNA encoding a transcription coactivator xSRC-3 by using retinoid X receptor (RXR) as a bait in the yeast two-hybrid screening. It belongs to a growing coactivator family that includes a steroid receptor coactivator amplified in breast cancer (AIB1), p300/ CREB-binding protein (CBP)-interacting protein (p/ CIP), and transcriptional intermediate factor 2 (TIF2). It also interacts with a series of nuclear receptors including retinoic acid receptor (RAR), thyroid hormone receptor (TR), and orphan nuclear receptors [hepatocyte nuclear receptor 4 (HNF4) and constitutive androstane receptor (CAR)]. However, it does not interact with small heterodimer partner (SHP), an orphan nuclear receptor known to antagonize ligand-dependent transactivation of other nuclear receptors. In CV-1 cells, cotransfection of xSRC-3 differentially stimulates ligand-induced transactivation of RXR, TR, and RAR in a dose-dependent manner. Interestingly, xSRC-3 is highly expressed in adult liver and early stages of oocyte development, suggesting that studies of xSRC-3 may lead to better understanding of the roles nuclear receptors play in oocyte development as well as liver-specific gene expression.

• Authors: H J Kim, S K Lee, S Y Na, H S Choi, J W Lee
• Journal: Molecular endocrinology (Baltimore, Md.) (Mol Endocrinol) Vol. 12 Issue 7 Pg. 1038-47 (Jul 1998) ISSN: 0888-8809 [Print] United States
• PMID: 9658407 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• NCOA2 protein, human
• NCOA3 protein, Xenopus
• Nuclear Receptor Coactivator 2
• Oncogene Proteins
• Receptors, Retinoic Acid
• Receptors, Thyroid Hormone
• Recombinant Fusion Proteins
• Retinoid X Receptors
• Trans-Activators
• Transcription Factors
• Xenopus Proteins
• Acetyltransferases
• Histone Acetyltransferases
• NCOA1 protein, human
• NCOA3 protein, human
• Nuclear Receptor Coactivator 1
• Nuclear Receptor Coactivator 3

Topics

• Acetyltransferases
• Alternative Splicing
• Amino Acid Sequence
• Animals
• Binding Sites
• Cell Nucleus (metabolism)
• Cloning, Molecular
• Female
• Gene Expression
• Histone Acetyltransferases
• Humans
• Molecular Sequence Data
• Mutagenesis
• Nuclear Receptor Coactivator 1
• Nuclear Receptor Coactivator 2
• Nuclear Receptor Coactivator 3
• Oncogene Proteins
• Receptors, Retinoic Acid (metabolism)
• Receptors, Thyroid Hormone (metabolism)
• Recombinant Fusion Proteins
• Retinoid X Receptors
• Saccharomyces cerevisiae (genetics)
• Sequence Homology
• Trans-Activators (chemistry, genetics, metabolism)
• Transcription Factors (chemistry, metabolism)
• Transcriptional Activation
• Xenopus
• Xenopus Proteins