Interleukin-12 p40 mRNA expression in bovine leukemia virus-infected animals: increase in alymphocytosis but decrease in persistent lymphocytosis.

Interleukin-12 (IL-12), a key cytokine in immune regulation, has an important role in activating the cell-mediated immune response in infectious diseases. Recently, a dichotomy between IL-12 and IL-10 regarding progression of a variety diseases has emerged. IL-12 activates type 1 cytokine production and has an antagonistic effect on type 2 cytokines. Here, by using quantitative competitive PCR, we show that peripheral blood mononuclear cells from bovine leukemia virus-infected animals in the alymphocytotic stage of disease express an increased amount of IL-12 p40 mRNA. In contrast, IL-12 p40 mRNA expression by cells from animals with late-stage disease, termed persistent lymphocytosis, was significantly decreased compared to that by normal and alymphocytotic animals. Interestingly, IL-12 p40 mRNA was also detected in tumor-bearing animals. IL-12 p40 expression occurred only in monocytes/macrophages, not B or T lymphocytes. The present study combined with previous findings suggest that IL-12 in bovine leukemia virus-infected animals may regulate production of other cytokines such as gamma interferon and IL-10 and the progression of bovine leukosis in animals that develop more advanced disease such as a persistent lymphocytosis of B cells or B-cell lymphosarcoma.
AuthorsD Pyeon, G A Splitter
JournalJournal of virology (J Virol) Vol. 72 Issue 8 Pg. 6917-21 (Aug 1998) ISSN: 0022-538X [Print] UNITED STATES
PMID9658146 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • RNA, Messenger
  • Interleukin-12
  • Animals
  • Cattle
  • Enzootic Bovine Leukosis (metabolism, physiopathology)
  • Female
  • Gene Expression
  • Interleukin-12 (biosynthesis, genetics)
  • Leukemia Virus, Bovine (physiology)
  • Lymphocytosis (metabolism, physiopathology)
  • Macrophages (metabolism)
  • Monocytes (metabolism)
  • RNA, Messenger
  • Virus Latency

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