Induction of apoptosis, p53 and heme oxygenase-1 by cytotoxic prostaglandin delta12-PGJ2 in transformed endothelial cells.

Abstract

Delta12-prostaglandin (PG)J2, which has been reported to have potent growth inhibitory activity in various tumor cells, induced apoptosis at 5 microg/ml culture medium in transformed mouse endothelial (F2) cells. Immunoblot analysis using anti-p53 or anti-WAF1 antibodies demonstrated that these two proteins had increased following delta12-PGJ2 treatment in F2 cells. Western blotting analysis using anti-heme oxygenase-1 (heat shock protein (HSP)32) antibody also revealed that delta12-PGJ2 induced HSP32 formation in F2 cells. HSP32 was also induced by heat shock treatment at 43 degrees C for 90 min. In contrast, HSP72 was not induced by heat shock or by delta12-PGJ2 treatment. In agreement with these findings, HSP32 immunofluorescence in the cytoplasm of F2 cells was intensified by delta12-PGJ2 treatment. More intense HSP32 immunoreactivity was similarly observed after heat shock treatment. These results suggest that delta12-PGJ2 caused the apoptotic cell death of F2 cells, which involved a certain process required for p53 or HSP32 induction.

• Authors: K Ikai, H Kudo, K Toda, M Fukushima
• Journal: Prostaglandins, leukotrienes, and essential fatty acids (Prostaglandins Leukot Essent Fatty Acids) Vol. 58 Issue 4 Pg. 295-300 (Apr 1998) ISSN: 0952-3278 [Print] Scotland
• PMID: 9654403 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antineoplastic Agents
• Heat-Shock Proteins
• Tumor Suppressor Protein p53
• 9-deoxy-delta-9-prostaglandin D2
• Oxygenases
• Heme Oxygenase (Decyclizing)
• Heme Oxygenase-1
• Hmox1 protein, rat
• Prostaglandin D2

Topics

• Animals
• Antineoplastic Agents (pharmacology)
• Apoptosis (drug effects)
• Blotting, Western
• Cell Line
• Cell Line, Transformed (cytology, drug effects, metabolism)
• DNA Fragmentation (drug effects)
• Endothelium, Vascular (cytology, drug effects, metabolism)
• Fluorescent Antibody Technique, Direct
• Heat-Shock Proteins (drug effects, immunology, metabolism)
• Heme Oxygenase (Decyclizing) (drug effects, metabolism)
• Heme Oxygenase-1
• Oxygenases
• Prostaglandin D2 (analogs & derivatives, pharmacology)
• Tumor Suppressor Protein p53 (drug effects, metabolism)