Abstract | OBJECTIVES: The activity of membrane-bound ecto-5'-nucleotidase and soluble e-Ns and c-N-II 5'-nucleotidases was evaluated on lymphocytes from patients affected by B-cell chronic lymphocytic leukemia (B-CLL). A statistically significative decrease in ecto-5'-nucleotidase, e-Ns, and c-N-II activities was observed in peripheral blood lymphocytes and in B and T populations from affected individuals. DESIGN AND METHODS: RESULTS: CONCLUSIONS: The decrease in ecto-5'-nucleotidase activity in patients with B-CLL is not due to loss of transcription of the specific mRNA. The presence of point mutations, splicing alteration, or posttranslational modifications must be investigated. If a defect at DNA or RNA level will be detected, the molecular analysis will be considered for diagnosis of B-cell chronic lymphocytic leukemia.
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Authors | F Rosi, A Tabucchi, F Carlucci, P Galieni, F Lauria, L Zanoni, R Guerranti, E Marinello, R Pagani |
Journal | Clinical biochemistry
(Clin Biochem)
Vol. 31
Issue 4
Pg. 269-72
(Jun 1998)
ISSN: 0009-9120 [Print] United States |
PMID | 9646951
(Publication Type: Journal Article)
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Chemical References |
- Isoenzymes
- RNA, Messenger
- 5'-Nucleotidase
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Topics |
- 5'-Nucleotidase
(blood, deficiency, genetics)
- Aged
- Blotting, Northern
- Enzyme Activation
- Fluorescent Antibody Technique
- Humans
- Isoenzymes
(blood, deficiency)
- Leukemia, Lymphocytic, Chronic, B-Cell
(blood, enzymology)
- Lymphocytes
(enzymology)
- Middle Aged
- Polymerase Chain Reaction
- RNA, Messenger
(analysis)
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