Abstract | OBJECTIVE: STUDY DESIGN: Flow cytometric analysis of nuclear DNA content was performed on paraffin-embedded tissues of 31 serous cystadenocarcinomas of the ovary. DNA ploidy, DNA index and S-phase fraction were determined without knowing the final outcome in the patients. RESULTS: Histograms in 14 (46%) cases were aneuploid, whereas 17 (54%) were diploid. Aneuploidy was more frequent at the advanced stages of the disease as well as in the poorly differentiated histologic types. However, ploidy had a prognostic influence: both the disease-free survival and overall survival were better in diploid than aneuploid tumors at early and advanced stages. S-phase fraction (SPF) could be correctly assessed in 19 cases (62%) and was > 15% in 21% (4 cases). The survival of those patients, however, was not significantly different from that of those with SPF < 15%. CONCLUSION: If used as an adjunct, DNA ploidy is of value in predicting tumor behavior, response to chemotherapy and disease recurrence.
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Authors | N Bakshi, A Rajwanshi, F Patel, N K Ganguly |
Journal | Analytical and quantitative cytology and histology
(Anal Quant Cytol Histol)
Vol. 20
Issue 3
Pg. 215-20
(Jun 1998)
United States |
PMID | 9642449
(Publication Type: Journal Article)
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Chemical References |
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Topics |
- Adult
- Cystadenocarcinoma, Serous
(diagnosis, genetics, pathology)
- DNA, Neoplasm
- Female
- Humans
- Middle Aged
- Ovarian Neoplasms
(diagnosis, genetics, pathology)
- Ploidies
- Prognosis
- S Phase
(genetics)
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