Human chondroitin 6-sulfotransferase: cloning, gene structure, and chromosomal localization.

Abstract

Chondroitin 6-sulfotransferase (C6ST) is the key enzyme in the biosynthesis of chondroitin 6-sulfate, a glycosaminoglycan implicated in chondrogenesis, neoplasia, atherosclerosis, and other processes. C6ST catalyzes the transfer of sulfate from 3'-phosphoadenosine 5'-phosphosulfate to carbon 6 of the N-acetylgalactosamine residues of chondroitin. Based on the previously published avian sequence, we searched the database of expressed sequence tags (dbEST) and obtained partial-length cDNAs that we completed by 5'-RACE using human chondrosarcoma and endothelial-cell RNA as template. Stable transfection of our full-length expression construct into CHO-K1 cells resulted in marked increases in C6ST and keratan sulfate sulfotransferase (KSST) enzymatic activities in cell homogenates. The predicted 411 amino acid sequence of human C6ST contains an N-terminal hydrophobic domain consistent with membrane insertion, four potential sites for N-linked glycosylation, several consensus sequences for protein phosphorylation, and one RGD sequence. The human and chick C6ST cDNA share 51% nucleotide identity, 40% amino acyl identity, and 75% amino acyl conservation. The human C6ST gene structure has been elucidated and exhibits an intron-less coding region, and the gene has been mapped to human chromosome 11 by radiation hybrid panel mapping.

• Authors: K D Mazany, T Peng, C E Watson, I Tabas, K J Williams
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 1407 Issue 1 Pg. 92-7 (Jul 01 1998) ISSN: 0006-3002 [Print] Netherlands
• PMID: 9639683 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• DNA, Complementary
• Phosphoadenosine Phosphosulfate
• Chondroitin
• Sulfotransferases
• carbohydrate sulfotransferases
• Acetylgalactosamine

Topics

• Acetylgalactosamine (metabolism)
• Amino Acid Sequence
• Base Sequence
• Chondroitin (metabolism)
• Chromosome Mapping
• Chromosomes, Human, Pair 11 (genetics)
• Cloning, Molecular
• DNA, Complementary (genetics)
• Gene Expression
• Humans
• Molecular Sequence Data
• Phosphoadenosine Phosphosulfate (metabolism)
• Sequence Analysis, DNA
• Sequence Homology, Amino Acid
• Sulfotransferases (genetics)