[Inhibitory effects of shimotsu-to, a traditional Chinese herbal prescription, on ultraviolet radiation-induced cell damage and prostaglandin E2 release in cultured Swiss 3T3 cells].

Abstract

We have investigated the effects of Shimotsu-to, a traditional Chinese herbal prescription (Kampo medicine), on ultraviolet (UV) radiation-induced cell damage and prostaglandin E2 (PGE2) release in cultured Swiss 3T3 cells to examine the anti-inflammatory mechanism of Shimotsu-to. Short-term UV irradiation significantly induced cell damage and stimulated PGE2 release in Swiss 3T3 cells cultured for 4 h. The UV-radiation-induced cell damage and the stimulation of PGE2 release were significantly suppressed by the treatment with Shimotsu-to. Among the single crude drug components of Shimotsu-to, Rehmanniae Radix showed significant protective effects against UV-radiation-induced cell damage and PGE2 release. Other synthetic anti-inflammatory agents, such as dexamethasone, dipotassium glycyrrhizinate and allantoin also protected against UV-induced cell damage and inhibited PGE2 release in cultured Swiss 3T3 cells. These results suggest that the anti-inflammatory mechanism of Shimotsu-to against UV-irradiated erythema (acute skin inflammation) in guinea pigs in vivo may be mediated by the inhibition of PGE2 release from cutaneous target cells.

• Authors: K Sakuma, M Ogawa, M Kimura, K Yamamoto, M Ogihara
• Journal: Yakugaku zasshi : Journal of the Pharmaceutical Society of Japan (Yakugaku Zasshi) Vol. 118 Issue 6 Pg. 241-7 (Jun 1998) ISSN: 0031-6903 [Print] Japan
• PMID: 9629060 (Publication Type: Journal Article)

Chemical References

• Anti-Inflammatory Agents
• Drugs, Chinese Herbal
• shimotsu-to
• Dinoprostone

Topics

• 3T3 Cells (metabolism, radiation effects)
• Animals
• Anti-Inflammatory Agents (pharmacology)
• Cells, Cultured
• Depression, Chemical
• Dinoprostone (metabolism)
• Drugs, Chinese Herbal (pharmacology)
• Mice
• Ultraviolet Rays