Glucose-6-phosphatase activity in the hypothalamus of the ob/ob mouse.

Abstract

The hypothalamus and cortex from ob/ob mice and their lean littermates were sonicated and then incubated with glucose-6-phosphate (glucose-6-P) and glycerol phosphate (glycerol-P). The difference between the rates of hydrolysis of glucose-6-P and glycerol-P was taken as the measure of glucose-6-phosphatase activity. The activity was much higher in the hypothalamus from ob/ob mice versus their lean littermates. Activity was undetected in the cortex. These findings raise the possibility that a defect in the regulation of glucose-6-phosphatase activity in a portion of the hypothalamus may relate to the mechanism underlying obesity in the ob/ob mouse. However, obese gene product administration to ob/ob mice, while reducing the body weight, did not alter the glucose-6-phosphatase activity.

• Authors: A Khan, L Zong-Chao, S Efendic, B R Landau
• Journal: Metabolism: clinical and experimental (Metabolism) Vol. 47 Issue 6 Pg. 627-9 (Jun 1998) ISSN: 0026-0495 [Print] United States
• PMID: 9627357 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Glycerophosphates
• Glucose-6-Phosphate
• alpha-glycerophosphoric acid
• Glucose-6-Phosphatase

Topics

• Animals
• Glucose-6-Phosphatase (metabolism)
• Glucose-6-Phosphate (metabolism)
• Glycerophosphates (metabolism)
• Hydrolysis
• Hypothalamus (enzymology)
• Mice
• Mice, Mutant Strains (genetics)
• Obesity (enzymology, genetics)
• Reference Values