Inhibition of fos-jun-DNA complex formation by dihydroguaiaretic acid and in vitro cytotoxic effects on cancer cells.

Abstract

The effect of dihydroguaiaretic acid (DHGA), isolated from the aryls of Myristica fragrans, on the transcription factor (fos-jun dimer) action was investigated via in vitro assay. DHGA showed an inhibitory effect against the complex formation of the fos-jun dimer and the DNA consensus sequence with an IC50 value of 0.21 micromol. Nordihydroguaiaretic acid (NDGA) and curcumin also inhibited fos-jun dimer action showing IC50 values of 7.9 and 6.9 nmol, respectively. DHGA and NDGA suppressed leukemia, lung cancer and colon cancer in an in vitro bioassay. The in vitro experiment suggested that inhibition of fos-jun-DNA complex formation could be due to the direct interference of fos-jun dimer binding onto the DNA consensus sequence by NDGA and curcumin.

• Authors: S Park, D K Lee, C H Yang
• Journal: Cancer letters (Cancer Lett) Vol. 127 Issue 1-2 Pg. 23-8 (May 15 1998) ISSN: 0304-3835 [Print] Ireland
• PMID: 9619854 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Lignans
• Proto-Oncogene Proteins c-fos
• Proto-Oncogene Proteins c-jun
• Transcription Factor AP-1
• dihydroguaiaretic acid
• Guaiacol
• Masoprocol

Topics

• Dimerization
• Guaiacol (analogs & derivatives, chemistry, pharmacology)
• Humans
• Lignans (chemistry, pharmacology)
• Masoprocol (pharmacology)
• Proto-Oncogene Proteins c-fos (drug effects)
• Proto-Oncogene Proteins c-jun (drug effects)
• Transcription Factor AP-1 (drug effects)
• Tumor Cells, Cultured (drug effects)