Down-regulation of phospholipase C-beta1 following chronic muscarinic receptor activation.

Abstract

To determine whether prolonged activation of a phospholipase C-coupled receptor can lead to a down-regulation of its effector enzyme, SH-SY5Y neuroblastoma cells were incubated for 24 h with the muscarinic receptor agonist, oxotremorine-M. Under these conditions, significant reductions (46-53%) in muscarinic cholinergic receptor density, G(alphaq/11) and phospholipase C-beta1 (but not the beta3-or gamma1 isoforms) were observed. These results suggest that a selective down-regulation of phospholipase C-beta1 may play a role in adaptation to chronic muscarinic receptor activation.

• Authors: S D Sorensen, D A Linseman, S K Fisher
• Journal: European journal of pharmacology (Eur J Pharmacol) Vol. 346 Issue 1 Pg. R1-2 (Apr 03 1998) ISSN: 0014-2999 [Print] Netherlands
• PMID: 9617763 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Isoenzymes
• Muscarinic Agonists
• Receptors, Muscarinic
• Oxotremorine
• oxotremorine M
• Type C Phospholipases
• PLCB1 protein, human
• PLCB3 protein, human
• Phospholipase C beta
• GTP-Binding Proteins

Topics

• Down-Regulation
• GTP-Binding Proteins (metabolism)
• Humans
• Isoenzymes (metabolism)
• Muscarinic Agonists (pharmacology)
• Oxotremorine (analogs & derivatives, pharmacology)
• Phospholipase C beta
• Receptors, Muscarinic (drug effects, metabolism)
• Tumor Cells, Cultured
• Type C Phospholipases (metabolism)