HOMEPRODUCTSCOMPANYCONTACTFAQResearchDictionaryPharmaSign Up FREE or Login

An evaluation of the usefulness of amylase isozyme differentiation in patients with hyperamylasemia.

Abstract
Amylase assays measure total activity without differentiating the relative contributions of pancreatic- and salivary-type amylase isozymes. Since polyacrylamide electrophoresis allows identification of salivary-and pancreatic-type isoxymes and their respective variants, serum and urine specimens from patients with the clinical diagnoses of mumps (4), pancreatitis (16), or undiagnosed hyperamylasemias (5) were compared with specimens from control subjects. Patients with mumps had elevations of salivary-type isozymes, while those with pancreatitis had elevations of pancreatic-type isozymes. Elevation of salivary-type isozymes was identified in the five patients who had undiagnosed hyperamylasemias; among these, the isozymes of two originated in neoplastic ovarian tissue and those of three, probably in the salivary glands. Amylase isozyme differentiation cannot unamibiguously identify the tissue source of hyperamylasemia. However, in patients whose hyperamylasemia is of unknown etiology or who respond atypically to therapy, amylase electrophoresis provides identification of the elevated isozyme type, thus providing the basis for the rational selection of further diagnostic procedures.
AuthorsL M Lehrner, J C Ward, R C Karn, C E Ehrlich, D Merritt
JournalAmerican journal of clinical pathology (Am J Clin Pathol) Vol. 66 Issue 3 Pg. 576-87 (Sep 1976) ISSN: 0002-9173 [Print] England
PMID961637 (Publication Type: Case Reports, Journal Article, Research Support, U.S. Gov't, P.H.S.)
Chemical References
  • Isoenzymes
  • Amylases
Topics
  • Aged
  • Amylases (blood, urine)
  • Child, Preschool
  • Electrophoresis, Polyacrylamide Gel
  • Female
  • Humans
  • Isoenzymes (blood, urine)
  • Male
  • Middle Aged
  • Mumps (diagnosis, enzymology)
  • Pancreas (enzymology)
  • Pancreatitis (diagnosis, enzymology)
  • Saliva (enzymology)

Join CureHunter, for free Research Interface BASIC access!

Take advantage of free CureHunter research engine access to explore the best drug and treatment options for any disease. Find out why thousands of doctors, pharma researchers and patient activists around the world use CureHunter every day.
Realize the full power of the drug-disease research graph!


Choose Username:
Email:
Password:
Verify Password:
Enter Code Shown: