Ascorbic acid recycling in N-myc amplified human neuroblastoma cells.

Abstract

The present study investigated the ability of two neuroblastoma cell lines (SK-N-SH, with one copy of N-myc, and SK-N-BE(2), with over 150 copies of N-myc) to recycle ascorbate by quantifying semidehydroascorbate reductase and dehydroascorbate reductase activities. Both cell lines expressed dehydroascorbate activity (SK-N-SH 28.4 +/- 9.8, SK-N-BE(2) 21.7 +/- 5.2 nmol/min/mg protein). Intracellular semidehydroascorbate activity was present only in SK-N-BE(2) cells (4.7 +/- 1.2 nmol/min/mg protein). Extracellular ascorbate was regenerated by semidehydroascorbate membrane activity, the activity of SK-N-BE(2) being twice that of SK-N-SH cells. The present data may explain the ability of the tumor to progress or regress through mechanisms involving both myc oncogene and apoptosis.

• Authors: I Savini, I D'Angelo, M Annicchiarico-Petruzzelli, L Bellincampi, G Melino, L Avigliano
• Journal: Anticancer research (Anticancer Res) 1998 Mar-Apr Vol. 18 Issue 2A Pg. 819-22 ISSN: 0250-7005 [Print] Greece
• PMID: 9615725 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• NAD
• Ascorbic Acid

Topics

• Ascorbic Acid (metabolism)
• Gene Amplification
• Genes, myc
• Humans
• NAD (metabolism)
• Neuroblastoma (genetics, metabolism)
• Oxidation-Reduction
• Tumor Cells, Cultured