Endogenous tumour necrosis factor-alpha sensitise melanoma cells to glucosaminylmuramyl dipeptide.

Abstract

Flow cytometry was used to demonstrate that cultured human melanoma BRO cells expressed membrane-bound tumour necrosis factor-alpha (TNF-alpha) and were able to release TNF-alpha upon treatment with glucosaminylmuramyl dipeptide (GMDP). The released TNF-alpha was shown to prime melanoma cells, previously unable to respond to GMDP by increasing expression of melanoma-associated antigens, making them sensitive to GMDP treatment.

• Authors: T I Valyakina, R L Komaleva, E E Petrova, A A Malakhov, O G Shamborant, T M Andronova, V A Nesmeyanov
• Journal: FEBS letters (FEBS Lett) Vol. 426 Issue 3 Pg. 373-6 (Apr 24 1998) ISSN: 0014-5793 [Print] England
• PMID: 9600269 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adjuvants, Immunologic
• Antigens, Neoplasm
• Culture Media, Conditioned
• Tumor Necrosis Factor-alpha
• Acetylmuramyl-Alanyl-Isoglutamine
• N-acetyl-beta-glucosaminyl-N-acetylmuramyl-alanylisoglutamine

Topics

• Acetylmuramyl-Alanyl-Isoglutamine (analogs & derivatives, pharmacology)
• Adjuvants, Immunologic (pharmacology)
• Animals
• Antigens, Neoplasm (biosynthesis, drug effects)
• Cell-Free System (physiology)
• Culture Media, Conditioned (pharmacology)
• Flow Cytometry
• Humans
• Melanoma (immunology, metabolism)
• Mice
• Tumor Cells, Cultured
• Tumor Necrosis Factor-alpha (physiology)