Cholera toxin-peroxidase: changes in surface labeling of glioblastoma cells with increased time in tissue culture.

Abstract

Cholera toxin coupled to peroxidase yielded a highly specific ultrastructural marker of plasma membrane monosialogangliosides. Studies with cultures of brain and brain tumors suggested that long-term culture of tissue in monolayers results in eventual loss of surface monosialogangliosides.

• Authors: L Manuelidis, E E Manuelidis
• Journal: Science (New York, N.Y.) (Science) Vol. 193 Issue 4253 Pg. 588-90 (Aug 13 1976) ISSN: 0036-8075 [Print] United States
• PMID: 959818 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Gangliosides
• Toxins, Biological
• Peroxidases

Topics

• Cell Line
• Cell Membrane (metabolism)
• Gangliosides (metabolism)
• Glioma (metabolism, pathology)
• Peroxidases
• Time Factors
• Toxins, Biological (metabolism)
• Vibrio cholerae