Anti-obesity effects of selective agonists to the beta 3-adrenergic receptor in dogs. II. Recruitment of thermogenic brown adipocytes and reduction of adiposity after chronic treatment with a beta 3-adrenergic agonist.

Abstract

The aim of this study was to evaluate the effectiveness of beta 3-adrenergic agonists for the treatment and prevention of obesity in the dog. When a selective beta 3-adrenergic agonist, CL316,243 (0.1 mg/kg), was given orally to adult beagles every day for 5-7 weeks, body weight and girth were decreased compared with control placebo-treated dogs. Gross anatomical examinations revealed no noticeable abnormalities in CL316,243-treated dogs, except an apparent decrease in abdominal fat. Immunohistochemical examination of perirenal adipose tissue showed a remarkable increase in brown adipocytes expressing a thermogenic protein, uncoupling protein (UCP). The increased expression of UCP and its mRNA in CL316,243-treated dogs was also confirmed by Western blot and reverse transcription polymerase chain reaction analyses. It was concluded that treatment with a beta 3-adrenergic agonist stimulates UCP expression, which may lead to an increase in energy expenditure, and thereby is useful for the treatment and prevention of obesity in the dog.

• Authors: N Sasaki, E Uchida, M Niiyama, T Yoshida, M Saito
• Journal: The Journal of veterinary medical science (J Vet Med Sci) Vol. 60 Issue 4 Pg. 465-9 (Apr 1998) ISSN: 0916-7250 [Print] Japan
• PMID: 9592719 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Adrenergic beta-Agonists
• Carrier Proteins
• Dioxoles
• Ion Channels
• Membrane Proteins
• Mitochondrial Proteins
• RNA, Messenger
• Receptors, Adrenergic, beta
• Receptors, Adrenergic, beta-3
• Uncoupling Protein 1
• disodium (R,R)-5-(2-((2-(3-chlorophenyl)-2-hydroxyethyl)-amino)propyl)-1,3-benzodioxole-2,3-dicarboxylate

Topics

• Adipose Tissue, Brown (drug effects, physiology)
• Adrenergic beta-Agonists (pharmacology)
• Animals
• Body Constitution
• Body Weight (drug effects)
• Carrier Proteins (biosynthesis)
• Dioxoles (pharmacology)
• Dog Diseases
• Dogs
• Female
• Gene Expression Regulation (drug effects)
• Ion Channels
• Male
• Membrane Proteins (biosynthesis)
• Mitochondrial Proteins
• Obesity (prevention & control, veterinary)
• RNA, Messenger (biosynthesis)
• Receptors, Adrenergic, beta (physiology)
• Receptors, Adrenergic, beta-3
• Transcription, Genetic (drug effects)
• Uncoupling Protein 1