Abstract |
Nanogram amounts of the proinflammatory cytokine interleukin-1alpha (IL-1alpha) were detected in uninfected cultures of human corneal epithelial cells ( HCEC). Although HSV-1 replicated >10(4)-fold in these cells and caused extensive cytopathic effects, virus infection was not accompanied by significant extracellular release of IL-1alpha. Additional studies showed that release of radiolabeled cytosolic proteins from virus-infected HCEC was no greater than that released by mock-infected cells. These findings indicate that HSV-1 infection of HCEC does not result in IL-1alpha release because newly formed virus progeny can escape infected cells without disrupting cell membranes.
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Authors | M T Tran, D A Dean, R N Lausch, J E Oakes |
Journal | Virology
(Virology)
Vol. 244
Issue 1
Pg. 74-8
(Apr 25 1998)
ISSN: 0042-6822 [Print] United States |
PMID | 9581780
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Interleukin-1
- Macromolecular Substances
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Topics |
- Cell Membrane
(physiology)
- Cell Membrane Permeability
- Cornea
(metabolism, physiology, virology)
- Epithelial Cells
(metabolism, physiology, virology)
- Herpesvirus 1, Human
(physiology)
- Humans
- Interleukin-1
(metabolism)
- Intracellular Fluid
- Macromolecular Substances
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