Abstract | BACKGROUND: METHODS: RESULTS:
Tumors from eight patients with post- transplantation lymphoproliferative disease, two patients with AIDS-associated non-Hodgkin's lymphoma, and three patients with endemic Burkitt's lymphoma were analyzed. Tumors from six of the patients with post- transplantation lymphoproliferative disease were positive for EBV and expressed LMP1; two samples were EBV-negative. Tumors from both patients with AIDS-associated non-Hodgkin's lymphoma were EBV-positive and expressed LMP1, whereas tumors from all three patients with Burkitt's tumors were positive for EBV but negative for LMP1. Double-immunofluorescence microscopy showed that LMP1 localized with and immunoprecipitated with TRAF-1 and TRAF-3 in all eight of the EBV-positive, LMP1-positive samples. An electrophoretic mobility shift assay revealed activated NF-kappaB in all eight EBV-positive, LMP1-positive samples as well, but not in either of the EBV-negative, LMP1-negative samples or in the three EBV-positive, LMP1-negative samples. CONCLUSIONS: LMP1-mediated signaling through the TRAF system has a role in the pathogenesis of the EBV-positive lymphomas that arise in immunosuppressed patients.
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Authors | D Liebowitz |
Journal | The New England journal of medicine
(N Engl J Med)
Vol. 338
Issue 20
Pg. 1413-21
(May 14 1998)
ISSN: 0028-4793 [Print] United States |
PMID | 9580648
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- EBV-associated membrane antigen, Epstein-Barr virus
- NF-kappa B
- Proteins
- TNF Receptor-Associated Factor 1
- TNF Receptor-Associated Factor 3
- Viral Matrix Proteins
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Topics |
- Acquired Immunodeficiency Syndrome
(complications)
- Gene Expression
- Herpesvirus 4, Human
(genetics, physiology)
- Humans
- Immunocompromised Host
- Lymphoma, Non-Hodgkin
(etiology, metabolism, virology)
- Lymphoproliferative Disorders
(etiology, metabolism, virology)
- Microscopy, Fluorescence
- NF-kappa B
(metabolism)
- Proteins
(physiology)
- Signal Transduction
- TNF Receptor-Associated Factor 1
- TNF Receptor-Associated Factor 3
- Transplantation
(adverse effects)
- Viral Matrix Proteins
(physiology)
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