Abstract |
Globo H (Fuc alpha1 --> 2Galbeta1 --> 3GalNAcbeta1 --> 3Gal alpha1 --> 4Galbeta1 --> 4Glc) is a carbohydrate structure that shows enhanced expression in many human carcinomas. From mice immunized with a globo H-KLH ( keyhole limpet hemocyanin) synthetic conjugate an IgG3 monoclonal antibody (mAb VK-9) was derived that recognizes the globo H structure. Serological analysis showed that the minimal structure recognized by this mAb was the tetrasaccharide sequence Fuc alpha1 --> 2Galbeta1 --> 3GalNAcbeta1 --> 3Gal. An isomeric structure with an internal alphaGalNAc linkage was also recognized but less efficiently. mAb VK-9 did not react with many related structures, such as galactosylgloboside, globoside, H type 1, H type 2 blood group structures or fucosyl-gangliotetraosyl ceramide, but did react weakly with globo A ceramide. Not only did mAb VK-9 react with carbohydrate- protein conjugates but it could also recognize globo H- ceramide and human tumor cells expressing globo H. These results suggest that globo H-KLH could be explored as a vaccine in the treatment of carcinoma patients.
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Authors | V Kudryashov, G Ragupathi, I J Kim, M E Breimer, S J Danishefsky, P O Livingston, K O Lloyd |
Journal | Glycoconjugate journal
(Glycoconj J)
Vol. 15
Issue 3
Pg. 243-9
(Mar 1998)
ISSN: 0282-0080 [Print] United States |
PMID | 9579801
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies, Monoclonal
- Antigens, Tumor-Associated, Carbohydrate
- Cancer Vaccines
- Glycoconjugates
- Immunoglobulin G
- Vaccines, Conjugate
- Hemocyanins
- keyhole-limpet hemocyanin
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Topics |
- Animals
- Antibodies, Monoclonal
(immunology)
- Antibody Specificity
- Antigens, Tumor-Associated, Carbohydrate
(chemistry)
- Cancer Vaccines
(chemistry, isolation & purification)
- Carbohydrate Sequence
- Female
- Glycoconjugates
(chemistry, immunology)
- Hemocyanins
(immunology)
- Humans
- Immunization
- Immunoglobulin G
(immunology)
- Mice
- Molecular Sequence Data
- Vaccines, Conjugate
(chemistry, isolation & purification)
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