[The mechanism of the effect of the cholinesterase reactivator diethyxime on different models of the development of delayed hypersensitivity in acute dichloroethane poisoning].

Abstract

Formation of DTH to sheep red blood cells was studied in CBA mice on different models in acute DCE poisoning (0.75 LD50). The effect of the reactivator cholinesterase diethyxime on this reaction in acute poisoning, and the relation of the reactions under study in various experimental series with the activity of alpha-naphthyl-AS-acetatesterase in the spleen cells and popliteal lymph nodes were also studied. Acute DCE poisoning (0.75 LD50) lowered cellular immunity which was assessed according to DTH formation in various models due to decrease of alpha-naphthyl-AS-acetatesterase activity in the splenocytes and cells of the of the suppressor cells in the DTH reaction. The reaction was attended with decrease of alpha-naphthyl-AS-acetatesterase in the splenocytes of the donors and the popliteal lymph nodes of the recipients apparently due to relative prevalence in the spleen cells of T-suppressors which are distinguished by poor esterase activity. Diethyxime restored completely the formation of DTH studied in different models (with the exception of the reaction in which the effect of transfer of the suppressor cells was assessed) due to restoration of alpha-naphthyl-AS-acetatesterase activity in the cells of the lymphoid organs.

• Authors: P F Zabrodskiĭ, V F Kirichuk, A V Gryzunov
• Journal: Eksperimental'naia i klinicheskaia farmakologiia (Eksp Klin Farmakol) 1998 Jan-Feb Vol. 61 Issue 1 Pg. 57-9 ISSN: 0869-2092 [Print] Russia (Federation)
• Vernacular Title: Mekhanizm vliianiia reaktivatora kholinésterazy diétiksima na razlichnye modeli formirovaniia giperchuvstvitel'nosti zamedlennogo tipa pri ostroĭ intoksikatsii dikhlorétanom.
• PMID: 9575415 (Publication Type: Comparative Study, English Abstract, Journal Article)

Chemical References

• Cholinesterase Reactivators
• Ethylene Dichlorides
• Oximes
• diethyxime

Topics

• Acute Disease
• Animals
• Cholinesterase Reactivators (therapeutic use)
• Disease Models, Animal
• Drug Evaluation, Preclinical
• Ethylene Dichlorides (poisoning)
• Hypersensitivity, Delayed (chemically induced, drug therapy, immunology)
• Immunity, Cellular (drug effects)
• Mice
• Mice, Inbred CBA
• Oximes (therapeutic use)
• Poisoning (complications, drug therapy, immunology)
• Spleen (drug effects, immunology)
• Time Factors