[Sairei-to inhibits the production of endothelin-1 by nephritic glomeruli(2): alisols, possible candidates as active compounds].

Abstract

We have previously reported that Sairei-to (TJ-114), a Japanese herbal medicine, prevented the production of endothelin-1 in anti-GBM nephritic rats, and that Alismatis Rhizoma (Takusha in Japanese), one of the twelve herbs composing TJ-114, might be responsible for the action. In order to further clarify the antinephritic components of TJ-114, we investigated the effects of Takusha extracts on various parameters, including endothelin-1 production of glomeruli in vitro and in vivo using anti-GBM nephritic rats. MeOH-100% MeOH and MeOH-50% MeOH fractions (31.3 microgram/ml or higher) strongly inhibited an increase in endothelin-1 concentration in culture medium when they were added to a culture of glomerular cells derived from nephritic rats. In addition, oral administration of the MeOH-100% MeOH fraction (30 mg/kg) ameliorated the proteinuria, increase in systolic blood pressure and changes in histopathological parameters in nephritic rats. Oral administration of the MeOH-100% MeOH fraction inhibited increase in endothelin-1 expression in the glomeruli of nephritic rats and in endothelin-1 production by a culture of glomerular cells derived from the nephritic rats. Alisols A and B, the main constituents of the MeOH-100% MeOH fraction, inhibited in vitro endothelin-1 production by glomerular cells derived from the nephritic rats. Oral administration of alisol B (30 mg/kg) prevented the endothelin-1 expression by glomeruli and the increase in endothelin-1 production by cultured nephritic glomerular cells. Oral administration of alisol B also ameliorated the proteinuria, the increase in systolic blood pressure and the changes in histopathological parameters in the nephritic rats. These results indicate that the antinephritic action of TJ-114, resulting from the inhibition of endothelin-1 production, may be attributed to the alisols in Takusha.

• Authors: T Hattori, H Nishimura, B Makino, S Shindo, H Kawamura
• Journal: Nihon Jinzo Gakkai shi (Nihon Jinzo Gakkai Shi) Vol. 40 Issue 2 Pg. 33-41 (Feb 1998) ISSN: 0385-2385 [Print] Japan
• PMID: 9567069 (Publication Type: English Abstract, Journal Article)

Chemical References

• Drugs, Chinese Herbal
• Endothelin-1
• sairei-to

Topics

• Animals
• Cells, Cultured
• Depression, Chemical
• Drugs, Chinese Herbal (pharmacology, therapeutic use)
• Endothelin-1 (biosynthesis)
• Glomerulonephritis (drug therapy, metabolism, pathology)
• Kidney Glomerulus (metabolism, pathology)
• Male
• Rats
• Rats, Sprague-Dawley