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Molecular cloning of a cDNA encoding molt-inhibiting hormone of the crab, Cancer magister.

Abstract
A neuropeptide molt-inhibiting hormone (MIH) negatively regulates crustacean molting glands (Y-organs). We report here the molecular cloning of a cDNA encoding putative MIH of the Dungeness crab, Cancer magister. A cDNA library was commercially prepared using poly (A+) RNA isolated from C. magister eyestalk neural ganglia. The library was screened using as probe a previously cloned portion of a cDNA encoding MIH of the blue crab, Callinectes sapidus. DNA sequence analysis of one positive clone revealed a 339 base pair open reading frame encoding a 78 amino acid putative MIH and a 35 amino acid signal peptide. The deduced amino acid sequence of C. magister MIH shows high sequence identity (80-98%) with MIH of three other brachyuran crabs, but lower identity (26-45%) with MIH and MIH-like peptides from astacurans and shrimp. Studies using reverse transcription-polymerase chain reaction (RT-PCR) indicate the MIH gene is expressed in eyestalk but not control (muscle, gill, gonad, hepatopancreas) tissue.
AuthorsH R Umphrey, K J Lee, R D Watson, E Spaziani
JournalMolecular and cellular endocrinology (Mol Cell Endocrinol) Vol. 136 Issue 2 Pg. 145-9 (Jan 15 1998) ISSN: 0303-7207 [Print] IRELAND
PMID9548218 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't, Research Support, U.S. Gov't, Non-P.H.S.)
Chemical References
  • DNA, Complementary
  • Invertebrate Hormones
  • Protein Sorting Signals
  • molt-inhibiting hormone
Topics
  • Amino Acid Sequence
  • Animals
  • Base Sequence
  • Brachyura (genetics)
  • Cloning, Molecular
  • Consensus Sequence
  • DNA, Complementary (chemistry, genetics)
  • Gene Library
  • Invertebrate Hormones (genetics)
  • Molecular Sequence Data
  • Protein Sorting Signals (chemistry)
  • Sequence Homology

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