Chemokine synthesis in the HSV-1-infected cornea and its suppression by interleukin-10.

Abstract

Herpes simplex virus type 1 (HSV-1) infection of the murine cornea results in a tissue-destructive inflammatory response. In this study we show that virus infection induces the synthesis of macrophage inflammatory protein-2 (MIP-2), MIP-1alpha, and monocyte chemoattractant protein-1 (MCP-1). However, only the production of MIP-2 and MIP-1alpha coincided with the influx of leukocytes into the cornea. IL-10 treatment markedly suppressed chemokine message and protein synthesis in vivo. Local administration of IL-10 also dramatically reduced the number of T cells and neutrophils migrating into the cornea and suppressed the severity of corneal disease. The inflammatory response could also be suppressed by the passive transfer of neutralizing antibody to MIP-1alpha but not MCP-1. We conclude that local IL-10 administration can suppress chemokine synthesis, thereby ameliorating corneal disease. Furthermore, our results indicate that MIP-1alpha plays a major role in herpes stromal keratitis development, whereas MCP-1 does not.

• Authors: T M Tumpey, H Cheng, X T Yan, J E Oakes, R N Lausch
• Journal: Journal of leukocyte biology (J Leukoc Biol) Vol. 63 Issue 4 Pg. 486-92 (Apr 1998) ISSN: 0741-5400 [Print] United States
• PMID: 9544579 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Antibodies
• Chemokine CCL2
• Chemokine CCL3
• Chemokine CCL4
• Chemokine CXCL2
• Chemokines
• Macrophage Inflammatory Proteins
• Monokines
• Interleukin-10

Topics

• Animals
• Antibodies (pharmacology)
• CD4-Positive T-Lymphocytes (cytology, drug effects)
• Cells, Cultured
• Chemokine CCL2 (immunology, physiology)
• Chemokine CCL3
• Chemokine CCL4
• Chemokine CXCL2
• Chemokines (metabolism)
• Chemotaxis, Leukocyte (drug effects)
• Cornea (drug effects, metabolism)
• Female
• Herpesvirus 1, Human (immunology)
• Immunization, Passive
• Immunohistochemistry
• Interleukin-10 (pharmacology)
• Keratitis, Herpetic (metabolism)
• Macrophage Inflammatory Proteins (immunology, physiology)
• Mice
• Mice, Inbred BALB C
• Monokines (physiology)
• Polymerase Chain Reaction
• Time Factors