Abstract | BACKGROUND: METHODS: In the present study, we evaluated the effects of A4.6.1 (100 microg twice weekly, i.p.) on growth and angiogenic activity of spheroids of the human prostatic cell line DU 145 (diameter 700 microm at implantation) implanted in dorsal skinfold chambers in nude mice (n = 11). An antibody of the same isotype (n = 5) or saline (n = 5) was used as control. Tumor cells were prelabeled with a fluorescent vital dye ( CMTMR), which allowed measurement of size of the implanted tumor spheroids throughout a two week observation period. FITC-dextran was used for plasma enhancement to visualize angiogenic activity. RESULTS:
Tumors of control animals induced a neo-vasculature with high vascular density (350+/-12 cm[-1]). In animals treated with the anti- VEGF antibody, there was complete inhibition of neovascularization of the micro tumors and complete inhibition of tumor growth after the initial prevascular angiogenesis independent growth phase. CONCLUSIONS: These results demonstrate that inhibition of the key regulatory paracrine growth factor for endothelial cells, VEGF, results in complete suppression of prostate cancer induced angiogenesis and prevents tumor growth beyond the initial prevascular growth phase.
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Authors | P Borgström, M A Bourdon, K J Hillan, P Sriramarao, N Ferrara |
Journal | The Prostate
(Prostate)
Vol. 35
Issue 1
Pg. 1-10
(Apr 01 1998)
ISSN: 0270-4137 [Print] United States |
PMID | 9537593
(Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)
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Chemical References |
- Antibodies, Monoclonal
- Endothelial Growth Factors
- Fluorescent Dyes
- Lymphokines
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factors
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Topics |
- Animals
- Antibodies, Monoclonal
(pharmacology, therapeutic use)
- Endothelial Growth Factors
(antagonists & inhibitors, immunology)
- Fluorescent Dyes
- Humans
- Lymphokines
(antagonists & inhibitors, immunology)
- Male
- Mice
- Mice, Nude
- Microscopy, Fluorescence
- Neoplasm Transplantation
- Neovascularization, Pathologic
(prevention & control)
- Prostatic Neoplasms
(blood supply, pathology)
- Tumor Cells, Cultured
- Vascular Endothelial Growth Factor A
- Vascular Endothelial Growth Factors
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