Expression of a receptor protein tyrosine phosphatase in human glial tumors.

Abstract

We have analyzed expression of a receptor protein tyrosine phosphatase (RPTPzeta/beta) in tissue samples from 23 human gliomas. Using the reverse transcription-polymerase chain reaction (RT-PCR) technique, we assayed for the presence or absence of mRNA transcripts encoding the intact receptor and 2 alternatively spliced forms of RPTPzeta/beta. Transcripts encoding the intact and truncated receptors were expressed in all of the lower grade gliomas (WHO grade 1-3) analyzed, but not in 55% of the grade 4 glioblastomas multiforme (GBM). However, this subset of GBMs did express an alternatively spliced secreted form comprised of only the RPTPzeta/beta extracellular domain. Our data suggests there may be a correlation between the loss of transcripts encoding the receptor forms of RPTPzeta/beta and progression from low to high grade gliomas. This work provides additional evidence for the importance of phosphatase isoform expression in human tumors.

• Authors: S A Norman, J G Golfinos, A C Scheck
• Journal: Journal of neuro-oncology (J Neurooncol) Vol. 36 Issue 3 Pg. 209-17 (Feb 1998) ISSN: 0167-594X [Print] United States
• PMID: 9524099 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Biomarkers, Tumor
• Nerve Tissue Proteins
• RNA, Messenger
• Receptors, Cell Surface
• PTPRZ1 protein, human
• Protein Tyrosine Phosphatases
• Receptor-Like Protein Tyrosine Phosphatases, Class 5

Topics

• Adolescent
• Adult
• Aged
• Animals
• Astrocytoma (enzymology)
• Biomarkers, Tumor (biosynthesis)
• Child
• Female
• Glioblastoma (enzymology)
• Glioma (enzymology)
• Humans
• Male
• Middle Aged
• Nerve Tissue Proteins (biosynthesis, genetics)
• Oligodendroglioma (enzymology)
• Protein Tyrosine Phosphatases (biosynthesis, genetics)
• RNA, Messenger (biosynthesis)
• Receptor-Like Protein Tyrosine Phosphatases, Class 5
• Receptors, Cell Surface (biosynthesis, genetics)