Abstract |
The subcellular localization of Bcl-2 protein in surgically resected, fixed-frozen tissue specimens of seven tumors from six cases of synovial sarcoma and a synovial sarcoma cell line was examined using laser-scanning confocal microscopy and immunoelectron microscopy. Bcl-2 protein in synovial sarcoma cells was detected in the nuclear envelope, endoplasmic reticulum membrane, and mitochondrial circumference. Based on the finding of pre-embedding immunoelectron-microscopy observation, the distribution of Bcl-2 protein in such membranous organella was patchy. A computer-based image analysis failed to reveal any quantitative differences between the inner and the outer mitochondrial membranes. The tumorigenesis of synovial sarcoma is also discussed from the viewpoint of Bcl-2 overexpression.
|
Authors | S Kawauchi, S Noguchi, T Fukuda, M Tsuneyoshi |
Journal | Pathology international
(Pathol Int)
Vol. 47
Issue 12
Pg. 854-61
(Dec 1997)
ISSN: 1320-5463 [Print] Australia |
PMID | 9503467
(Publication Type: Journal Article)
|
Chemical References |
- Proto-Oncogene Proteins c-bcl-2
|
Topics |
- Adult
- Blotting, Western
- Female
- Fluorescent Antibody Technique, Indirect
- Humans
- Immunohistochemistry
- Male
- Microscopy, Confocal
- Microscopy, Immunoelectron
- Middle Aged
- Proto-Oncogene Proteins c-bcl-2
(metabolism)
- Sarcoma, Synovial
(metabolism, ultrastructure)
- Tumor Cells, Cultured
|