A simple dipstick assay for the detection of antibodies to phenolic glycolipid-I of Mycobacterium leprae.

Abstract

Among the many reported applications of the detection of antibodies to phenolic glycolipid-I (PGL-I) of Mycobacterium leprae, in particular, the use of seroprevalence as an indicator of the magnitude of the leprosy problem may turn out to be very useful in leprosy control programs. An operational function of serology within the leprosy control services requires a simple test system. We have developed a simple dipstick assay for the detection of antibodies to PGL-I and compared its performance with that of an ELISA. A high degree of agreement (97.2%) was observed between the ELISA and the dipstick assay when tested on 435 sera; the agreement beyond chance (Kappa value) was 0.92. No significant difference was found between the dipstick assay and the ELISA when seropositivity rates obtained in groups of leprosy patients, household contacts, and controls were compared. The interpretation of the dipstick results as positive or negative was unequivocal, as illustrated by the high agreement between different persons reading the test (Kappa values > 0.88). Storage of the only reagents required, the dipsticks and the stabilized detection reagent, up to three weeks under tropical conditions of high temperatures, high humidity, and exposure to light, did not influence the results of the assay. The dipstick assay described here is an easy-to-perform method for the detection of IgM antibodies to PGL-I of M. leprae; it does not require any special equipment and the highly stable reagents make the test robust and suitable for use in tropical countries. An internal control validates the performance of the assay. This dipstick assay may be the method of choice for epidemiologic mapping of leprosy.

• Authors: S S Bührer, H L Smits, G C Gussenhoven, C W van Ingen, P R Klatser
• Journal: The American journal of tropical medicine and hygiene (Am J Trop Med Hyg) Vol. 58 Issue 2 Pg. 133-6 (Feb 1998) ISSN: 0002-9637 [Print] United States
• PMID: 9502593 (Publication Type: Comparative Study, Journal Article, Research Support, Non-U.S. Gov't)

Chemical References

• Antibodies, Bacterial
• Antigens, Bacterial
• Glycolipids
• Immunoglobulin M
• Reagent Strips
• phenolic glycolipid I, Mycobacterium leprae

Topics

• Antibodies, Bacterial (blood)
• Antigens, Bacterial (immunology)
• Enzyme-Linked Immunosorbent Assay
• Glycolipids (immunology)
• Hot Temperature
• Humans
• Humidity
• Immunoglobulin M (blood)
• Leprosy (diagnosis, epidemiology, immunology)
• Light
• Mycobacterium leprae (immunology)
• Philippines (epidemiology)
• Preservation, Biological
• Reagent Strips
• Reproducibility of Results
• Time Factors