Formation in vitro of ascorbic acid 2-sulfate.

Abstract

The sulfation of ascorbic acid by an ascorbic acid sulphotransferase was investigated using rat liver and colon homogenates. When Na2 35 SO4 or 3'-phosphoadenylyl [35S]sulfate (P-Ado-P-35S) and ascorbic acid were used as substrates, chromatographic behavior of the reaction products on thin-layer cellulose suggested that ascorbic acid 2-[35S]sulfate was formed. With Na2 35SO4 as the source of radioactive sulfate in the assay system, ATP was found to be an obligatory cofactor. Incorporation of [35S]sulfate frofrom Na2 35SO4 into ascorbic acid 2-[35S]sulfate was also decreased when ATP sulfurylase inhibitors were added to the system. P-Ado-O35S alone in the assay without ATP was an extemely effective sulfating agent. In addition, liver and colon homogenates from vitamin A deficient and sufficient rats were used in one of the studies. Vitamin A deficiency appeared to have little effect on ascorbic acid 2-sulfate formation.

• Authors: M Mohamram, R B Rucker, R E Hodges
• Journal: Biochimica et biophysica acta (Biochim Biophys Acta) Vol. 437 Issue 1 Pg. 305-10 (Jun 23 1976) ISSN: 0006-3002 [Print] Netherlands
• PMID: 949509 (Publication Type: Journal Article, Research Support, U.S. Gov't, P.H.S.)

Chemical References

• Sulfuric Acids
• Sulfurtransferases
• Ascorbic Acid

Topics

• Animals
• Ascorbic Acid (metabolism)
• Colon (metabolism)
• Kinetics
• Liver (metabolism)
• Organ Specificity
• Rats
• Sulfuric Acids (metabolism)
• Sulfurtransferases (metabolism)
• Vitamin A Deficiency (metabolism)