Abstract |
We attempted to study the possible relationships between neutrophil-type procollagenase/pro- matrix metalloproteinase (MMP-8) and the serine proteinases plasmin, cathepsin G and tryptase in bronchiectasis. The presence of the plasmin/ plasminogen system and plasmin-, cathepsin G- and tryptase-like activities were compared to the activity of endogenously activated MMP-8 in bronchoalveolar lavage (BAL) fluid in 38 bronchiectasis patients and in 14 healthy controls by means of immunohistochemistry, Western-blot and substrate-based functional assays. In contrast to cathepsin G- and tryptase-like activities, the plasmin/ plasminogen activator system in BAL fluid was observed to have a relatively weak activation stage and no correlation with disease severity. Neither plasmin-like activities nor concentrations of plasminogen activators from the bronchiectatic patients differed significantly from the values of healthy controls. Immunolocation of plasminogen activator inhibitor-1 showed a marked, but not significant, increase in bronchiectatic lung as compared to controls. In contrast to cathepsin G- and tryptase-like activities, with their strong and significant correlation with endogenously activated collagenase (r=0.9; p=0.0001; and r=0.6; p=0.03, respectively), no correlations were observed between plasmin-like and endogenously activated collagenase (r=0.3; p=0.2) in bronchiectasis. These findings suggest that cathepsin G- and tryptase-like activities may act as potent pro- matrix metalloproteinase-8 activators in patients with bronchiectasis, whereas the plasminogen activator/ plasmin cascade was shown to be down-regulated.
|
Authors | R Sepper, Y T Konttinen, L Buø, K K Eklund, A Lauhio, T Sorsa, H Tschesche, A O Aasen, H Sillastu |
Journal | The European respiratory journal
(Eur Respir J)
Vol. 10
Issue 12
Pg. 2788-93
(Dec 1997)
ISSN: 0903-1936 [Print] England |
PMID | 9493662
(Publication Type: Clinical Trial, Controlled Clinical Trial, Journal Article, Research Support, Non-U.S. Gov't)
|
Chemical References |
- Inflammation Mediators
- Cathepsins
- Serine Endopeptidases
- chymase 2
- CTSG protein, human
- Cathepsin G
- Chymases
- Tryptases
- Fibrinolysin
- Collagenases
- Matrix Metalloproteinase 8
|
Topics |
- Adolescent
- Adult
- Analysis of Variance
- Biopsy
- Blotting, Western
- Bronchiectasis
(diagnosis, enzymology, pathology)
- Bronchoalveolar Lavage Fluid
(chemistry)
- Cathepsin G
- Cathepsins
(analysis)
- Chymases
- Collagenases
(analysis)
- Down-Regulation
- Female
- Fibrinolysin
(analysis)
- Humans
- Immunohistochemistry
- In Vitro Techniques
- Inflammation Mediators
(analysis)
- Lung
(enzymology, pathology)
- Male
- Matrix Metalloproteinase 8
- Neutrophil Activation
- Serine Endopeptidases
(analysis)
- Tryptases
|