Abstract |
Human naive and germinal center (GC) B cells were sorted by flow cytometry and rearranged VH region genes were amplified and sequenced from single cells. Whereas no deletions or insertions were found in naive B cells, approximately 4% of in-frame and >40% of out-of-frame rearrangements of GC B cells harbored deletions and/or insertions of variable length. The pattern of deletions/insertions and their restriction to mutated V genes strongly suggests that they result from somatic hypermutation. Deletions and insertions account for approximately 6% of somatic mutations introduced into rearranged VH region genes of GC B cells. These deletions/insertions seem to be the main cause for the generation of heavy chain disease proteins. Furthermore, it appears that several types of oncogene translocations (like c-myc translocations in Burkitt's lymphoma) occur as a byproduct of somatic hypermutation within the GC-and not during V(D)J recombination in the bone marrow as previously thought.
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Authors | T Goossens, U Klein, R Küppers |
Journal | Proceedings of the National Academy of Sciences of the United States of America
(Proc Natl Acad Sci U S A)
Vol. 95
Issue 5
Pg. 2463-8
(Mar 03 1998)
ISSN: 0027-8424 [Print] United States |
PMID | 9482908
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Immunoglobulin Heavy Chains
- Immunoglobulin Variable Region
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Topics |
- B-Lymphocytes
(immunology)
- Base Sequence
- Flow Cytometry
- Gene Deletion
- Gene Rearrangement, B-Lymphocyte, Heavy Chain
- Genes, Immunoglobulin
- Heavy Chain Disease
(genetics, immunology)
- Hodgkin Disease
(genetics)
- Humans
- Immunoglobulin Heavy Chains
(genetics)
- Immunoglobulin Variable Region
(genetics)
- Lymphoma, Non-Hodgkin
(genetics)
- Molecular Sequence Data
- Multigene Family
- Oncogenes
- Polymerase Chain Reaction
- Translocation, Genetic
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