Abstract |
Ribosomes have different conformations in cells that are starved for a required amino acid (giving aminoacyl. tRNA starvation), or treated with kirromycin (blocking EF-Tu. GDP release), or are in exponential growth. A tunnel spans the 50S ribosome from a location facing the 70S ribosomal intersubunit space to the back side of the subunit in Escherichia coli cells. Here we have analyzed the internal low density region that corresponds to this tunnel in ribosomes in vivo. The data suggest that the tunnel is opened in connection with spatial separation of the subunits in ribosomes that have an empty A-site due to starvation for aminoacyl. tRNA. A region that corresponds to this tunnel can be found in the more compact structure of ribosomes in kirromycin-treated cells only after a substantial removal of low density material. This region is even less prominent in ribosomes in undefined working modes in growing bacteria. The data suggest that appearance of the tunnel through the 50S ribosomal subunit is working-mode dependent and it is not a characteristic feature of the major fraction of the ribosomal population in growing cells.
|
Authors | K Zhang, L G Ofverstedt, L Pettersson-Landén, U Skoglund, L A Isaksson |
Journal | Experimental cell research
(Exp Cell Res)
Vol. 238
Issue 2
Pg. 345-53
(Feb 01 1998)
ISSN: 0014-4827 [Print] United States |
PMID | 9473342
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
|
Copyright | Copyright 1998 Academic Press. |
Chemical References |
- Anti-Bacterial Agents
- Pyridones
- Tryptophan
- mocimycin
|
Topics |
- Anti-Bacterial Agents
(pharmacology)
- Escherichia coli
(drug effects, ultrastructure)
- Image Processing, Computer-Assisted
(methods)
- Microscopy, Electron
(methods)
- Pyridones
(pharmacology)
- Ribosomes
(ultrastructure)
- Tryptophan
(physiology)
|