Abstract |
Recently, a chlorophyll-related compound, pheophytin a, has been identified from an edible green alga, Enteromorpha prolifera (Sujiao-nori in Japanese) as a potent suppressive substance against genotoxin-induced umu C gene expression in a tester bacteria (Okai and Higashi-Okai, 1997, J. Sci. Food Agricul. 71, 531-535). In the present study, anti-inflammatory effects of pheophytin a from Enteromorpha prolifera have been analyzed using in vitro and in vivo experiments. 1. Pheophytin a suppressed the production of superoxide anion (O2-) in mouse macrophages induced by 12-O-tetradecanoylphorbol-13-acetate (TPA) using the cytochrome C reduction method. 2. Pheophytin a caused a suppressive effect against formyl- Met-Leu-Phe, (FMLP)-induced chemotaxis of human polymorphonuclear leukocytes (PMNs) in Boyden's chamber experiment. 3. Pheophytin a exhibited a significant suppression against TPA-induced inflammatory reaction such as edema formation in BALB/c mouse ear. These results suggest that pheophytin a from Enteromorpha prolifera has a potent anti-inflammatory activity.
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Authors | Y Okai, K Higashi-Okai |
Journal | International journal of immunopharmacology
(Int J Immunopharmacol)
Vol. 19
Issue 6
Pg. 355-8
(Jun 1997)
ISSN: 0192-0561 [Print] England |
PMID | 9467755
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Chemical References |
- Anti-Inflammatory Agents
- Pheophytins
- Superoxides
- N-Formylmethionine Leucyl-Phenylalanine
- pheophytin a
- Tetradecanoylphorbol Acetate
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Topics |
- Animals
- Anti-Inflammatory Agents
(isolation & purification, pharmacology)
- Chlorophyta
(chemistry)
- Edema
(pathology)
- Humans
- In Vitro Techniques
- Macrophages
(drug effects, metabolism)
- Mice
- Mice, Inbred BALB C
- N-Formylmethionine Leucyl-Phenylalanine
(pharmacology)
- Pheophytins
(isolation & purification, pharmacology)
- Superoxides
(metabolism)
- Tetradecanoylphorbol Acetate
(pharmacology)
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