Abstract | BACKGROUND: This study describes the impact of prolonged cold storage on microvascular reperfusion injury of transplanted rat small bowel isografts. METHODS: In vivo fluorescence microscopy was used to assess intestinal microcirculation after 6, 12, 18 and 24 h of cold (4 degrees C) ischaemia in University of Wisconsin solution and 20-90 min of reperfusion. Sham-operated animals served as controls. RESULTS: Whereas 6 and 12 h of ischaemia did not affect functional capillary density of the intestinal graft mucosa, villous perfusion was significantly impaired after 18 and 24 h of cold preservation. Similarly, microvascular perfusion of circular and longitudinal muscle was not affected after 6 h, but deteriorated following prolonged cold ischaemia. Leucocyte-endothelial cell interaction in submucosal venules was significantly enhanced after 6 h of ischaemia with peak values after 12 and 18 h. A progressive reduction of lymphatic capillary drainage indicated an ischaemia time-related deterioration in graft function. CONCLUSION: The results provide evidence that leucocyte-endothelial cell interaction in submucosal venules of the transplanted intestine is a primary step in the manifestation of reperfusion injury following short periods of cold ischaemia.
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Authors | S Massberg, A P Gonzalez, R Leiderer, M D Menger, K Messmer |
Journal | The British journal of surgery
(Br J Surg)
Vol. 85
Issue 1
Pg. 127-33
(Jan 1998)
ISSN: 0007-1323 [Print] England |
PMID | 9462404
(Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
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Topics |
- Animals
- Cryopreservation
- Intestine, Small
(blood supply, transplantation)
- Male
- Microcirculation
- Microscopy, Electron
- Rats
- Rats, Inbred Lew
- Reperfusion Injury
(prevention & control)
- Transplantation, Isogeneic
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