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In vivo assessment of the influence of cold preservation time on microvascular reperfusion injury after experimental small bowel transplantation.

AbstractBACKGROUND:
This study describes the impact of prolonged cold storage on microvascular reperfusion injury of transplanted rat small bowel isografts.
METHODS:
In vivo fluorescence microscopy was used to assess intestinal microcirculation after 6, 12, 18 and 24 h of cold (4 degrees C) ischaemia in University of Wisconsin solution and 20-90 min of reperfusion. Sham-operated animals served as controls.
RESULTS:
Whereas 6 and 12 h of ischaemia did not affect functional capillary density of the intestinal graft mucosa, villous perfusion was significantly impaired after 18 and 24 h of cold preservation. Similarly, microvascular perfusion of circular and longitudinal muscle was not affected after 6 h, but deteriorated following prolonged cold ischaemia. Leucocyte-endothelial cell interaction in submucosal venules was significantly enhanced after 6 h of ischaemia with peak values after 12 and 18 h. A progressive reduction of lymphatic capillary drainage indicated an ischaemia time-related deterioration in graft function.
CONCLUSION:
The results provide evidence that leucocyte-endothelial cell interaction in submucosal venules of the transplanted intestine is a primary step in the manifestation of reperfusion injury following short periods of cold ischaemia.
AuthorsS Massberg, A P Gonzalez, R Leiderer, M D Menger, K Messmer
JournalThe British journal of surgery (Br J Surg) Vol. 85 Issue 1 Pg. 127-33 (Jan 1998) ISSN: 0007-1323 [Print] England
PMID9462404 (Publication Type: Journal Article, Research Support, Non-U.S. Gov't)
Topics
  • Animals
  • Cryopreservation
  • Intestine, Small (blood supply, transplantation)
  • Male
  • Microcirculation
  • Microscopy, Electron
  • Rats
  • Rats, Inbred Lew
  • Reperfusion Injury (prevention & control)
  • Transplantation, Isogeneic

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